Biological Fluorescence Detection Based on Aggregation of Donor-acceptor Dyes

2016 Fiscal Year Final Research Report

• Project/Area Number: 26410105
• Research Category: Grant-in-Aid for Scientific Research (C)
• Allocation Type: Multi-year Fund
• Section: 一般
• Research Field: Functional solid state chemistry
• Research Institution: Kurume National College of Technology
• Principal Investigator: ISHI-I Tsutomu 久留米工業高等専門学校, 生物応用化学科, 教授 (60346856)
• Research Collaborator: TOBITA Seiji
• Project Period (FY): 2014-04-01 – 2017-03-31
• Keywords: 発光 / 生体検出 / ドナー・アクセプター / 糖質分解酵素

Outline of Final Research Achievements

Fluorescence detection of glycosidase was investigated on the basis of aggregate emission of benzothiadiazole-triphenylamine donor-acceptor dyes. The parent donor-acceptor dye, which is fluorescent only in the aggregate state, indicated a decrease of emission intensity by introducing galactopyranoside moiety. In contrast, the emission intensity was recovered and enhanced upon addition of galactosidase, which reacted with the galactopyranoside moiety to release the fluorescent parent donor-acceptor dye, leading to the emission enhancement in the aggregate state. As a result of the emission enhancement, the galactosidase detection can be achieved.

Free Research Field

化学