Development of Gel Filtration Chromatography technique for the Purification of Surface-functionalized Gold Nanoparticles

2016 Fiscal Year Final Research Report

• Project/Area Number: 26410142
• Research Category: Grant-in-Aid for Scientific Research (C)
• Allocation Type: Multi-year Fund
• Section: 一般
• Research Field: Analytical chemistry
• Research Institution: Akita University
• Principal Investigator: OGAWA NOBUAKI 秋田大学, その他部局等, 理事 (80169193)
• Project Period (FY): 2014-04-01 – 2017-03-31
• Keywords: 金属ナノ粒子 / サイズ排除クロマトグラフィー / 表面修飾

Outline of Final Research Achievements

Surface functionality of gold nanoparticles (AuNPs) with small organic compounds such as thiols and surfactants or biomolecules allows to control their surface properties. Thus, the surface functionalization and purification of the functionalized AuNPs form residual modification reagents are key processes in biological and therapeutic applications. Here, we devise a novel versatile methodology for surface functionalization of AuNPs through a purification of functionalized-AuNPs by using a gel filtration chromatography (GFC). We tested purification of a surface-functionalized AuNPs by bovine serum albumin (BSA)-and three different charged thiols (11-mercapto-1-undecanoic acid, 11-amino-1-undecane thiol and 1-undecane thiol), respectively. The surface-functionalized AuNPs were successfully separated with high recovery rates of more over than 90% for BSA and 85% for thiols.

Free Research Field

分析化学