Development of HPLC columns for ultra high performance separation of bio-based highly hydrophilic compounds

2016 Fiscal Year Final Research Report

• Project/Area Number: 26410151
• Research Category: Grant-in-Aid for Scientific Research (C)
• Allocation Type: Multi-year Fund
• Section: 一般
• Research Field: Analytical chemistry
• Research Institution: Kyoto Institute of Technology
• Principal Investigator: Ikegami Tohru 京都工芸繊維大学, 分子化学系, 准教授 (20301252)
• Project Period (FY): 2014-04-01 – 2017-03-31
• Keywords: 親水性相互作用クロマトグラフィー / 液体クロマトグラフィー / キラル分離 / カラム充填剤 / 分離特性解析

Outline of Final Research Achievements

High performance / highly selective hydrophilic interaction (HILIC) type, ion exchange type, chiral separation type HPLC columns were prepared for monolithic and particle-packed type. They provided highly hydrophilic nature exceeding the performance limit of commercially available HILIC columns. (1) The relationship between the reaction conditions of the polymerization for surface modification of silica and the corresponding performance of the column were carefully examined to establish optimal modification conditions. (2) The correlation between the chain length of the introduced polymer chains and the separation performance and characteristics were examined. (3) HILIC type columns providing theoretical plate height 4-10 microns, 300,000 theoretical plates were prepared and applied to metabolome analysis. (4) A column having all of HILIC, cation exchange, anion exchange and chiral separation ability was prepared.

Free Research Field

分析化学