Development of PCR-free electrochemical detection of micro RNAs

2016 Fiscal Year Final Research Report

• Project/Area Number: 26410155
• Research Category: Grant-in-Aid for Scientific Research (C)
• Allocation Type: Multi-year Fund
• Section: 一般
• Research Field: Analytical chemistry
• Research Institution: Kyushu Institute of Technology
• Principal Investigator: Sato Shinobu 九州工業大学, 大学院工学研究院, 准教授 (80510677)
• Research Collaborator: HAYASHIDA Yasunobu ; NAKAYAMA Akira ; ONO Mizuki
• Project Period (FY): 2014-04-01 – 2017-03-31
• Keywords: miRNA / 電気化学遺伝子検出 / フェロセン化ナフタレンジイミド / DNA・RNA2本鎖

Outline of Final Research Achievements

It is known that micro RNAs (miRNAs) are associated with an expression of tumor suppressor genes and are expecting to be one of good cancer markers. Here, we developed ferrocenylnaphthalene diimide (FND)-based hybridization assay to analyze specific miRNA without any amplification step. Since our previous research demonstrated that FND can bind to DNA-RNA hetero duplex, we constructed miRNA detecting system using FND coupled with an electrode chip carrying DNA probe for target miRNA. Here, we chose the suitable FND derivatives (FND1-7) to detect the miRNA by using Tm measurement and try to detect of target miRNA by using electrochemical hybridization assay. FND3 has the highest stabilization ability for DNA-RNA duplex and gave an increasing current shift with target miRNA in this hybridization assay, whereas almost no current shift was observed with non-target miRNA.

Free Research Field

バイオ分析化学