2016 Fiscal Year Final Research Report
Pattern analysis of post-translational modification using on-line coupling of immunoadsorption and capillary isoelectric focusing.
| Project/Area Number |
26410158
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| Research Category |
Grant-in-Aid for Scientific Research (C)
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| Allocation Type | Multi-year Fund |
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| Section | 一般 |
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| Research Field |
Analytical chemistry
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| Research Institution | Fukushima Medical University |
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Principal Investigator |
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| Project Period (FY) |
2014-04-01 – 2017-03-31
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| Keywords | electrophoresis / isoelectric focusing / immunoadsorption / PTM / modform analysis / capillary |
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| Outline of Final Research Achievements |
A separation and analysis device that integrates an immunoadsorption column and a capillary for isoelectric focusing was developed. By using this device, an analysis method was developed to elute the sample trapped in the immunoadsorption column into the device and analyze the eluted sample by capillary isoelectric focusing. This method can efficiently separate and determine the charge variants of a protein produced by post-translational modifications.
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| Free Research Field |
バイオ分析化学
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