Development of an analytical method for metabolomics of fecal organic acid in intestinal flora

2016 Fiscal Year Final Research Report

• Project/Area Number: 26410161
• Research Category: Grant-in-Aid for Scientific Research (C)
• Allocation Type: Multi-year Fund
• Section: 一般
• Research Field: Analytical chemistry
• Research Institution: Tokyo University of Pharmacy and Life Science
• Principal Investigator: KOTANI Akira 東京薬科大学, 薬学部, 准教授 (40318184)
• Project Period (FY): 2014-04-01 – 2017-03-31
• Keywords: 有機酸 / 電気分析化学 / 液体クロマトグラフィー / 糞便 / メタボロミクス

Outline of Final Research Achievements

A high-performance liquid chromatography with electrochemical detection (HPLC-ECD) system based on a voltammetric reduction of quinone was developed to perform the metabolomics of fecal organic acids in intestinal flora. The present HPLC-ECD system performed the determination of fecal organic acids (lactic, acetic, propionic, butyric, isovaleric, and valeric acids) in rats. Moreover, a change of fecal organic acids in rats after ingestion of an antimicrobial agent and a Kampo medicine was monitored by the present HPLC-ECD system. It was shown that the present HPLC-ECD system was useful to examine the effect of foods and medicines on bioactivities of intestinal flora.

Free Research Field

分析化学