2017 Fiscal Year Final Research Report
Inovation in fluorous chemistry focusing on selective analysis of peptides and proteins
| Project/Area Number |
26410166
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| Research Category |
Grant-in-Aid for Scientific Research (C)
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| Allocation Type | Multi-year Fund |
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| Section | 一般 |
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| Research Field |
Analytical chemistry
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| Research Institution | Fukuoka University |
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Principal Investigator |
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| Co-Investigator(Renkei-kenkyūsha) |
YAMAGUCHI Masatoshi 福岡大学, 薬学部, 教授 (50117280)
HAYAMA Tadashi 福岡大学, 薬学部, 准教授 (90549693)
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| Project Period (FY) |
2014-04-01 – 2018-03-31
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| Keywords | フルオラス化学 / 前処理 / ペプチド / タンパク質 / 誘導体化 / アミノ基 / リン酸基 |
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| Outline of Final Research Achievements |
Highly selective and effective purification methods for peptides and proteins have been developed. One is for polyamino-containing peptides. The amino moieties of peptides are perfluoroalkylated with amine-reactive fluorous reagent, then the derivatives are highly selectively extracted to a fluorous solvent, followed by LC/MS/MS analysis. The other is for phosphorylated peptides. The Fe(III) immobilized fluorous reagent is designed and synthesized. The reagent reacts with phosphorylated peptides to form their fluorous chelates, those are extracted selectively into a fluorous solvent. The former is applied to the determination of brain peptides in rat brain samples, and the latter to the assay for protein kinase activity.
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| Free Research Field |
分析化学
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