2016 Fiscal Year Final Research Report
DNA methylation analysis utilizing rotation of mismatched base
| Project/Area Number |
26410168
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| Research Category |
Grant-in-Aid for Scientific Research (C)
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| Allocation Type | Multi-year Fund |
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| Section | 一般 |
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| Research Field |
Analytical chemistry
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| Research Institution | National Institute of Advanced Industrial Science and Technology |
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Principal Investigator |
KURITA RYOJI 国立研究開発法人産業技術総合研究所, バイオメディカル研究部門, 研究グループ長 (50415676)
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| Project Period (FY) |
2014-04-01 – 2017-03-31
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| Keywords | エピジェネティクス / マイクロ流路分析 / イムノアッセイ |
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| Outline of Final Research Achievements |
We proposed a new analytical method to detect the methylation state of cytosine in DNA utilizing the rotation of mismatched DNA bases, and evaluated properties and fabricated a micro-sensing device. Conventionally, although the total amount of methylcytosine can be measured with antibody, the utility value is poor due to unknown position information in the DNA sequence. We challenged sequence-selective antibody recognition utilizing outward turned mismatched bases and rapid measurement by micro-sensing device fabrication. We have developed a novel SPR chip (epigenetic chip) to realize methylation assessment of oligo- and genomic- DNAs in a small SPR instrument.
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| Free Research Field |
分析化学
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