2016 Fiscal Year Final Research Report
Development of fluorogenic probe for gene-analisis utilizing novel silylated fluorescent material
| Project/Area Number |
26410173
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| Research Category |
Grant-in-Aid for Scientific Research (C)
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| Allocation Type | Multi-year Fund |
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| Section | 一般 |
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| Research Field |
Bio-related chemistry
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| Research Institution | Gunma University |
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Principal Investigator |
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| Co-Investigator(Renkei-kenkyūsha) |
MORIGUCHI Tomohisa 群馬大学, 大学院理工学府, 准教授 (70334133)
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| Research Collaborator |
Jakir Ahmed Chowdury
KAWASAKI Masakazu
IRIE Yusuke
HIDA Satomi
MOKI Daisuke
TOKITA Kazuki
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| Project Period (FY) |
2014-04-01 – 2017-03-31
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| Keywords | 蛍光性オリゴDNA / シリル化ピレン / シリル化ペリレン / モレキュラービーコン / 一塩基ミスマッチ |
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| Outline of Final Research Achievements |
Novel pyrene and perylene derivatives bearing a silly functional group were synthesized and incorporated into oligoDNA . The silylated pyrene and perylene derivatives possess enhanced fluorescence signal may be due to the characteristic sigma-pai interaction brought about by the silly group. The obtained oligoDNA baring a silylated pyrene at its 5'-terminus has a partial self-complementary sequence and, the fore, forms dumbbell shaped higher structure. In a buffer solution, the oligoDNA exhibited a faint fluorescence signal while it stays alone. On the other hand, the signal of the modified DNA showed a marked recovery (10 to 40 times) under the presence of the complementary oligonucleotide. In an appropriate conditions the modified DNA gave fluorescence signal under the presence of one-base mismatched complements in same extent as that while it stays alone. Thus, the modified DNA discriminated one-base mismatched complements with showing a fluorescence signal.
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| Free Research Field |
生物有機化学
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