Development of life-survey methods using SERS probes

2016 Fiscal Year Final Research Report

• Project/Area Number: 26410179
• Research Category: Grant-in-Aid for Scientific Research (C)
• Allocation Type: Multi-year Fund
• Section: 一般
• Research Field: Bio-related chemistry
• Research Institution: Kyoto Institute of Technology
• Principal Investigator: KOBORI AKIO 京都工芸繊維大学, 分子化学系, 准教授 (00397605)
• Project Period (FY): 2014-04-01 – 2017-03-31
• Keywords: SERS / ラマン散乱 / DNA / RNA

Outline of Final Research Achievements

It is reported that concentration of particular RNAs in body fluids is associated with the onset of the diseases. Therefore the RNAs in blood serum, urine, and saliva are anticipated to be used as biomarkers for the diagnosis of severe diseases. Although reverse transcription quantitative PCR is generally used to quantify RNAs, the PCR has been poorly utilized as a diagnostic method due to a complicated operation for amplification of nucleic acids. Until now, we have developed a highly sensitive and simple nucleic acid detection system that does not require the amplification step of nucleic acids. In this study, we developed a chemical tag with a Raman signal in a region that does not appear any signals of the biological component in the Raman spectrum and constructed a sandwich assay system using a SERS positive tag.

Free Research Field

生体関連化学