Introduction of lactam with useful functions into proteins at specific lysine residue by reactions with artificial nucleic acids

2016 Fiscal Year Final Research Report

• Project/Area Number: 26410181
• Research Category: Grant-in-Aid for Scientific Research (C)
• Allocation Type: Multi-year Fund
• Section: 一般
• Research Field: Bio-related chemistry
• Research Institution: Kyushu University
• Principal Investigator: Aso Mariko 九州大学, 薬学研究院, 准教授 (30201891)
• Co-Investigator(Renkei-kenkyūsha): KATAYAMA Tsutomu 九州大学, 大学院薬学研究院, 教授 (70264059)
• Project Period (FY): 2014-04-01 – 2017-03-31
• Keywords: 核酸 / 蛋白質 / リジン / ラクタム / 蛍光

Outline of Final Research Achievements

We developed oligodeoxynucleotides (ODNs) containing 1,4-dicarbonyl groups to modify specific lysine residue in DNA-interacting proteins and provide information on position and function of the lysine residue in question. Reductive amination of ODNs containing 1,4-dicarbonyl groups in ribose moiety proceeded with a proximal lysine residue in protein-ODN complex and cross-link products were obtained efficiently. ODNs containing 1,4-dicarbonyl groups on substituted benzene ring modified a proximal lysine residue to produce stable fluorescent compound and turn-on fluorescent protein modification at a specific lysine residue was achieved.

Free Research Field

核酸関連化学