Quantification of post-translational modified amino acids by 2D-HPLC and ultra-sensitive amino acid analysis

2016 Fiscal Year Final Research Report

• Project/Area Number: 26410190
• Research Category: Grant-in-Aid for Scientific Research (C)
• Allocation Type: Multi-year Fund
• Section: 一般
• Research Field: Bio-related chemistry
• Research Institution: National Institute of Maritime, Port and Aviation Technology
• Principal Investigator: Masuda Akiko 国立研究開発法人海上・港湾・航空技術研究所, その他部局等, 研究員 (10322679)
• Project Period (FY): 2014-04-01 – 2017-03-31
• Keywords: アミノ酸分析 / 翻訳後修飾 / タンパク質

Outline of Final Research Achievements

Proteins are chemically modified after translation based on genetic information of DNA, the modifications which are related to the expression of various functions of proteins. The modifications are referred to as post-translational modifications (PTMs). It is important to elucidate the relationship between PTMs and protein functions. In order to analysis of PTMs, modified amino acids themselves must be quantified since there is no description in DNA of what kind of modifications are executed. Until now, however, it has been difficult to quantify the modified amino acids because only small amounts of them are contained. In this study, quantitative hydrolysis method of proteins to amino acids and 2-dimensional HPLC to improve the resolution of analysis were developed. Combining these techniques with ultra-sensitive amino acid analysis, the analysis method was established for absolute quantification of modified amino acids.

Free Research Field

分析化学