2016 Fiscal Year Final Research Report
An Investigation on a Light Waveguide Implemented Optical Total Analysis System for Internal Cell Structure Analysis
| Project/Area Number |
26420210
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| Research Category |
Grant-in-Aid for Scientific Research (C)
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| Allocation Type | Multi-year Fund |
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| Section | 一般 |
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| Research Field |
Intelligent mechanics/Mechanical systems
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| Research Institution | Toyo University |
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Principal Investigator |
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| Project Period (FY) |
2014-04-01 – 2017-03-31
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| Keywords | TAS（総合分析システム） / マイクロ流路 / 光導波路 / 蛍光検出 / FDTD法 / BPM法 / 光スキャナー |
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| Outline of Final Research Achievements |
The purpose of our research is to estimate distribution of fluorescent substance inside cells or micro particles utilizing directivity of emitted fluorescence from such micro objects. In order to obtain precise directivity, we utilized a transparent resin material and fabricated it as cell-sized micro fluidic channels and light waveguides formed on a monolithic chip. We prepared three stages of development steps: they were, to obtain extremely weak fluorescence with higher signal-to-noise ratio, to obtain fluorescence by switching light waveguides sequentially, and finally, to detect fluorescence by changing illuminating angle sequentially. Three goals could have almost been achieved, and as a result, we have succeeded in obtaining directivity of a 5-μm-diameter resin particle with uniformly distributed fluorescent substance.
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| Free Research Field |
知能機械学・機械システム
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