2016 Fiscal Year Final Research Report
Application and characterization of Streptomyces SCMPpromoter
Project/Area Number |
26420805
|
Research Category |
Grant-in-Aid for Scientific Research (C)
|
Allocation Type | Multi-year Fund |
Section | 一般 |
Research Field |
Biofunction/Bioprocess
|
Research Institution | 岡山県農林水産総合センター生物科学研究所 |
Principal Investigator |
HATANAKA Tadashi 岡山県農林水産総合センター生物科学研究所, その他部局等, 専門研究員 (00344408)
|
Project Period (FY) |
2014-04-01 – 2017-03-31
|
Keywords | 放線菌 / ストレプトマイセス / プロモーター |
Outline of Final Research Achievements |
To determine the essential region working as a promoter, I constructed expression vectors including a secreted leucine aminopeptidase gene from Streptomyces sp. under the SCMP promotor, which was identified a powerful promoter from S. cinnamoneus TH-2. The vectors were containing of the first half, the last half, 63 bps from the 3’ termini and 43 bps from the 3’ termini of the SCMP promoter, respectively. By using S. lividans 1326 and above vectors, secreted aminopeptidase activities were compared. From the results, there is no working by using the first half of SCMP promoter. On the other hand, 63 bps from the 3’ termini showed a strong activity. Moreover, it is possible to use various expression media when the region was used as a promotor.
|
Free Research Field |
生化学
|