Development of biodosimetric method for medical triaging in radiation accident

2017 Fiscal Year Final Research Report

• Project/Area Number: 26420879
• Research Category: Grant-in-Aid for Scientific Research (C)
• Allocation Type: Multi-year Fund
• Section: 一般
• Research Field: Nuclear engineering
• Research Institution: National Institutes for Quantum and Radiological Science and Technology
• Principal Investigator: Suto Yumiko 国立研究開発法人量子科学技術研究開発機構, 放射線医学総合研究所 計測・線量評価部, チームリーダー(定常) (70313202)
• Co-Investigator(Kenkyū-buntansha): 穐山 美穂 国立研究開発法人量子科学技術研究開発機構, 放射線医学総合研究所 計測・線量評価部, 技術員(任常) (70415404)
• Project Period (FY): 2014-04-01 – 2018-03-31
• Keywords: 生物学的線量評価 / 緊急被ばく医療 / PCC法 / PNA-FISH

Outline of Final Research Achievements

Conventional cytogenetic assays require two- or three-day peripheral blood lymphocyte culture before starting chromosome analyses to estimate biological doses. Therefore, alternative technologies that suit requirements for triage biodosimetry are needed. Radiation-induced DNA double strand breaks in G0 lymphocytes can be detected as interphase chromosome aberrations by the cell fusion-mediated premature chromosome condensation (PCC) method. In the present study, in combination with PCC and fluorescence in situ hybridization using pan-centromeric and telomeric peptide nucleic acid probes, prematurely condensed dicentric chromosome (PCDC) assay was developed, which has the potential for evaluating exposed radiation doses in as short as six hours after the collection of peripheral blood specimens for triage mode biodosimetry.

Free Research Field

放射線生物学、人類遺伝学