2017 Fiscal Year Final Research Report
To reveal the mechanisms of cell-lineage dependent coritcal neural circuits formation during postnatal development
| Project/Area Number |
26430047
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| Research Category |
Grant-in-Aid for Scientific Research (C)
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| Allocation Type | Multi-year Fund |
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| Section | 一般 |
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| Research Field |
Nerve anatomy/Neuropathology
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| Research Institution | Osaka University (2017)
National Institute for Physiological Sciences (2014-2016) |
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Principal Investigator |
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| Project Period (FY) |
2014-04-01 – 2018-03-31
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| Keywords | 神経回路 |
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| Outline of Final Research Achievements |
We analyzed developmental establishment of the cell-lineage-dependent neural connections in mouse barrel cortex using chimeric mice which is generated by injection of GFP-expressing mouse-derived iPS cells.
There was no significant difference in the connection probability between clonal and non-clonal pairs at P9-11. The probability increased significantly from P9-11 to P14-16, then decreased only in clonal pairs, resulting in the same connection probability in clonal and non-clonal pairs at P18-20. The proportion of reciprocally connected pairs among connected pairs was not significantly different between clonal and non-clonal neuron pairs at P9-11. Then the reciprocity continued to increase significantly in clonal pairs until P18-20, whereas it showed only an insignificant increase in non-clonal pairs during that period. These results suggest that cell lineage dependent reciprocal connections are formed accompanied with cell lineage specific reconstruction of synaptic connectivity.
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| Free Research Field |
神経回路形成
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