Role of spine-resident transcriptional coactivator in dendritic spine formation

2016 Fiscal Year Final Research Report

• Project/Area Number: 26430063
• Research Category: Grant-in-Aid for Scientific Research (C)
• Allocation Type: Multi-year Fund
• Section: 一般
• Research Field: Neurochemistry/Neuropharmacology
• Research Institution: Gunma University
• Principal Investigator: Yamazaki Hiroyuki 群馬大学, 大学院医学系研究科, 助教 (10334137)
• Project Period (FY): 2014-04-01 – 2017-03-31
• Keywords: 樹状突起スパイン / 転写調節因子 / 養育行動 / マイクロアレイ

Outline of Final Research Achievements

Spikar is a transcriptional coactivator localized in dendritic spines and nucleus in neuron. We conducted real-time imaging of spikar in dendritic spines and analyses of spikar-KO mice. FRAP analysis showed that the dynamics of spikar in dendritic spine is regulated by actin-binding protein drebrin. In drebrin-knockdown neurons, the stable fraction of spikar was decreased, and the phenotype was rescued by drebrin-transfection. In the behavior analysis, we found abnormal maternal behaviors in spikar-KO mice. Spikar (-/-) females showed increased the time retrieving their pups and defect in grouping. Further, we analyzed transcriptome of spikar- KO mice brain by using microarray data. Gene ontology and pathway analyses revealed that the expression of numerous genes related with cytoskeleton and apoptosis signaling are regulated by spikar.

Free Research Field

分子神経生物学