Visualisation of the marker for oligodendrocytes by imaging mass spectrometry

2016 Fiscal Year Final Research Report

• Project/Area Number: 26430077
• Research Category: Grant-in-Aid for Scientific Research (C)
• Allocation Type: Multi-year Fund
• Section: 一般
• Research Field: Neurochemistry/Neuropharmacology
• Research Institution: Kansai Medical University
• Principal Investigator: WADA-HIRAHARA Yukie (平原幸恵) 関西医科大学, 医学部, 助教 (70457969)
• Research Collaborator: YAO Ikuko 浜松医科大学, メディカルフォトニクス研究センター 光イメージング研究室, 准教授 (60399681) ; HONKE Koichi 高知大学, 医学部, 教授 (80190263) ; ONO Katsuhiko 京都府立医科大学, 医学部, 教授 (30152523) ; YAMADA Hisao 関西医科大学, 医学部, 教授 (00142373)
• Project Period (FY): 2014-04-01 – 2017-03-31
• Keywords: スルファチド / プロオリゴデンドロブラスト / 質量顕微鏡 / ミエリン / 脂肪酸

Outline of Final Research Achievements

Sulfatide species comprise the major glycosphingolipid components of myelin. Although various sulfatides contain different fatty acids, it is unclear how these sulfatides affect myelination. The O4 antibody reaction with sulfatide has been widely used as a useful marker for oligodendrocytes/myelin. However, sulfatide synthesis during the pro-oligodendroblast stage has not been examined. Here, we identified a sulfatides from the pro-oligodendroblast to myelination stage by imaging mass spectrometry. The results demonstrated short-chain sulfatides with 16 carbon fatty acids (C16) and C18 existed in the area, where pro-oligodendroblasts initially appear. C22 sulfatide became predominant later in oligodendrocyte development and the longer C24 sulfatide was predominant in the adult brain. These findings indicated that O4 recognized sulfatides with short chain fatty acids in pro-oligodendroblasts and individual sulfatide species may have unique roles in oligodendrogenesis and myelination.

Free Research Field

神経化学