Systematic genetic analysis for urethane-induced lung cancer susceptibility loci in mice

2017 Fiscal Year Final Research Report

• Project/Area Number: 26430088
• Research Category: Grant-in-Aid for Scientific Research (C)
• Allocation Type: Multi-year Fund
• Section: 一般
• Research Field: Laboratory animal science
• Research Institution: Nagoya University
• Principal Investigator: Ohno Tamio 名古屋大学, 医学系研究科, 准教授 (90293620)
• Co-Investigator(Renkei-kenkyūsha): TOYOKUNI Shinya 名古屋大学, 大学院医学系研究科, 教授 (90252460) ; UNNO Akihiro 名古屋大学, 大学院医学系研究科, 助教 (50628592)
• Research Collaborator: MIYASAKA Yuki 名古屋大学, 大学院医学系研究科, 助教 (30778098) ; MAEGAWA Tomoki
• Project Period (FY): 2014-04-01 – 2018-03-31
• Keywords: マウス / 体系的遺伝解析系 / コンソミック系統 / コンジェニック系統 / ウレタン誘発肺腫瘍 / 肺腫瘍感受性遺伝子

Outline of Final Research Achievements

QTL analysis using SMXA・RI strains revealed plumonary adenoma resistance locus 1 (Par1) on Chr.11 and Par3 on Chr.12. However, it could not be confirmed the existence and effects of these loci by using congenic strains developed in this study. We conducted re-mapping by the Chr. 11 congenic strains, and indicated that the other Par locus was located between 27.72Mb and 36.39Mb. The Mpg gene, which encodes a ubiquitous DNA repair enzyme associated with lung cancer, is located within this region. A Sanger sequence analysis of the Mpg gene revealed five polymorphic sites in the A/J genome. One variant, p.Ala132Ser, was suggested the strong candidate mutation with regard to susceptibility to urethan-induced lung cancer.

Free Research Field

実験動物学