2016 Fiscal Year Final Research Report
Treatment for fulminant hepatitis focusing on soluble receptor for HMGB1 (sRAGE)
| Project/Area Number |
26430093
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| Research Category |
Grant-in-Aid for Scientific Research (C)
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| Allocation Type | Multi-year Fund |
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| Section | 一般 |
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| Research Field |
Laboratory animal science
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| Research Institution | Keio University |
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Principal Investigator |
Shinoda Masahiro 慶應義塾大学, 医学部(信濃町), 准教授 (50286499)
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| Project Period (FY) |
2014-04-01 – 2017-03-31
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| Keywords | 急性肝不全 / HMGB1 / 可溶性受容体 |
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| Outline of Final Research Achievements |
High-mobility group box 1 (HMGB1) has recently been identified as an important mediator of various kinds of acute and chronic inflammation. Acute liver failure is a lethal disease. We constructed plasmid encoding cDNA for sRAGE and performed in vitro and in vivo experiments to test if the plasmid works for acute liver failure. Western blot analysis showed enhanced expressions of sRAGE protein in HeLa cells transfected with plasmid. The culture supernatant of HeLa cells transfected with the plasmid inhibited TNF production from macrophages. Transfected rats showed tendency of decreased hepatic enzymes, It is possible that sRAGE is effective for the treatment for acute liver failure.
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| Free Research Field |
臓器移植
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