Rescue of age-related meiotic segregation errors in mouse oocytes

2016 Fiscal Year Final Research Report

• Project/Area Number: 26430100
• Research Category: Grant-in-Aid for Scientific Research (C)
• Allocation Type: Multi-year Fund
• Section: 一般
• Research Field: Laboratory animal science
• Research Institution: Institute of Physical and Chemical Research
• Principal Investigator: Ogonuki Narumi 国立研究開発法人理化学研究所, バイオリソースセンター, 専任技師 (40373287)
• Project Period (FY): 2014-04-01 – 2017-03-31
• Keywords: マウス / 発生工学

Outline of Final Research Achievements

It is well known that low quality oocytes increases with maternal age in humans. Low quality oocytes cause low pregnancies, or chromosome abnormalities of newborn infants. In this study, the quality of aged oocyte improved by non-micromanipulating approaches using mice. Abnormal revel (high revel) of histone deacetylase (HDAC) and aneuploidy were seen in MII oocytes after knock-down with histone deacetylase (HDAC) activity with "young oocytes" (9- to 12-week-old). These phenotypes were similar to aged oocytes, so HDAC activity might be involved to aged oocyte abnormalities.　In superovulation methods of aged female mice (8- to 15-month-old), using injection of anti-inhibin serum (AIS) which works with endogenous FSH instead of eCG which works as exogenous FSH was increased the number of ovulated oocytes and decreased abnormal oocytes.

Free Research Field

発生工学