Analysis how circulating plasma DNA release to peripheral blood and that applies to metastatic animal model using human lung cancer cells

2016 Fiscal Year Final Research Report

• Project/Area Number: 26430144
• Research Category: Grant-in-Aid for Scientific Research (C)
• Allocation Type: Multi-year Fund
• Section: 一般
• Research Field: Tumor diagnostics
• Research Institution: Saga University
• Principal Investigator: Aragane Naoko 佐賀大学, 医学部, 准教授 (20321846)
• Co-Investigator(Renkei-kenkyūsha): KIMURA SHINYA 佐賀大学, 医学部, 教授 (80359794)
• Project Period (FY): 2014-04-01 – 2017-03-31
• Keywords: リキッドバイオプシー / バイオマーカー / 血漿DNA

Outline of Final Research Achievements

We established a highly metastatic animal model using immunodeficient mice inoculated with human lung cancer cell line, H1975 carrying EGFR mutations. Tumor-derived circulating DNA (ctDNA) was analyzed using MBP-QP method on the animal model, and the results are obtained as follows:1) Frequency of ctDNA detection was correlated with tumor burden and metastasis, suggesting that ctDNA appears in peripheral blood concomitant with tumor progression.2) Organ preference of metastasis was analyzed. Inoculation with H1975 and H226B was metastasized into lymph nodes and lung, respectively. Several molecules were more phosphorylated in metastatic lymph nodes compared to primary lesion.3) Monitoring of anti-tumor effect of 3rd generation EGFR tyrosine kinase inhibitor could be performed by detection of ctDNA.

Free Research Field

臨床腫瘍学