2016 Fiscal Year Final Research Report
Identification of biomarkers by RNAi screening that predict efficacy of chemotherapy in esophageal cancer
| Project/Area Number |
26430146
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| Research Category |
Grant-in-Aid for Scientific Research (C)
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| Allocation Type | Multi-year Fund |
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| Section | 一般 |
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| Research Field |
Tumor diagnostics
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| Research Institution | Jikei University School of Medicine |
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Principal Investigator |
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| Project Period (FY) |
2014-04-01 – 2017-03-31
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| Keywords | 食道癌 / 薬剤感受性 / 薬剤耐性 / RNAiスクリーニング |
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| Outline of Final Research Achievements |
In this study, we explored genes whose knockdown promotes esophageal cancer cells increased sensitivity or resistance to anticancer drugs using genome-wide RNA interference screening.
First, we performed positive-selection screening. We infected esophageal cancer cells (KYSE170) with shRNA pool and then treated cells with cisplatin. We obtained a dozen sublines from KYSE170 that were resistant to cisplatin. We identified genes whose inhibition rendered esophageal cells resistant to cisplatin.
Next, we performed negative-selection screening. We divided shRNA-infected KYSE170 cells into two groups and administered cisplatin to one group. Then we compared the abundance of each shRNA. We found that the inhibition of genes involved in nucleotide excision repair and histone modification increased cisplatin sensitivity of esophageal cancer cells.
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| Free Research Field |
臨床腫瘍学
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