2016 Fiscal Year Final Research Report
Highly sensitive detection of ubiquitination using tumor-bearing mouse model
| Project/Area Number |
26430147
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| Research Category |
Grant-in-Aid for Scientific Research (C)
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| Allocation Type | Multi-year Fund |
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| Section | 一般 |
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| Research Field |
Tumor diagnostics
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| Research Institution | Himeji Dokkyo University |
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Principal Investigator |
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| Project Period (FY) |
2014-04-01 – 2017-03-31
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| Keywords | ユビキチン化 / がん診断 / ユビキチンリガーゼ / E2活性 / ARF |
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| Outline of Final Research Achievements |
The ubiquitin-conjugating enzymes (E2) in ubiquitination are associated with various diseases such as leukemia, lung cancer, and breast cancer. Thus, assessing E2 activity leads to the monitoring of tumor development for diagnostic and prognostic purposes. Here, we described the detection of E2 activity on a signal accumulation ISFET biosensor (AMIS sensor) using an artificial RING finger (ARF). The use of ARF enabled the simplified detection of E2 activity without a substrate. The high-sensitivity quantitative detection of E2 activities was demonstrated via real-time monitoring over a response range of femtomolar to micromolar concentrations. Furthermore, the monitoring of E2 activities was successfully achieved using human acute promyelocytic leukemia cells following treatment with the anticancer drug bortezomib, which allowed the assessment of the pathological conditions.
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| Free Research Field |
生物分析化学
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