Molecular profiling of proprotein convertases toward identification of secreted pro-hormone precursor proteins as novel tumor markers

2016 Fiscal Year Final Research Report

• Project/Area Number: 26430150
• Research Category: Grant-in-Aid for Scientific Research (C)
• Allocation Type: Multi-year Fund
• Section: 一般
• Research Field: Tumor diagnostics
• Research Institution: Shizuoka Cancer Center Research Institute
• Principal Investigator: Ohshima Keiichi 静岡県立静岡がんセンター(研究所), その他部局等, 研究員 (10399587)
• Project Period (FY): 2014-04-01 – 2017-03-31
• Keywords: 腫瘍マーカー / プロホルモン変換酵素 / ELISA / セクレトーム / 培養細胞 / プロテオーム / エクソーム / トランスクリプトーム

Outline of Final Research Achievements

Prohormone is a protein precursor to produce a bioactive peptide by processing, in which prohormone convertases (PCs) are involved. Prohormone of gastric releasing peptide (GRP), proGRP, is abundant and stable in blood from small cell lung cancer patients, resulting in development of a tumor marker. The aim of this study is to identify a novel prohormone-derived tumor marker by estimating the processing function of PCs followed by proteomics-based identification of secreted prohormones. Using cancer cell lines derived from various tissues, including lung, stomach, and colon, along with clinical specimens of tumor and matched normal tissue and blood samples, we profiled gene expression, mutation status, and copy number change in 9 PC genes. This dataset is an invaluable resource for further identification of prohormones in the extracellular spaces. Using the dataset profiling all genes, we also clarified genes with amplification-dependent overexpression to predict cancer driver genes.

Free Research Field

腫瘍生物学