Comprehensive functional analysis of colorectal cancer biomarker candidates for the exploitation of diagnostic and therapeutic methods

2016 Fiscal Year Final Research Report

• Project/Area Number: 26430152
• Research Category: Grant-in-Aid for Scientific Research (C)
• Allocation Type: Multi-year Fund
• Section: 一般
• Research Field: Tumor diagnostics
• Research Institution: Osaka University (2016); National Institutes of Biomedical Innovation, Health and Nutrition (2014-2015)
• Principal Investigator: Hara Yasuhiro 大阪大学, 医学系研究科, 特任研究員(常勤) (70568617)
• Project Period (FY): 2014-04-01 – 2017-03-31
• Keywords: 大腸癌 / 膜タンパク質 / バイオマーカー

Outline of Final Research Achievements

We previously reported the identification of a number of membrane proteins which were increased in colorectal cancer tissues. These proteins appeared to be biomarker candidates for colorectal cancer. In this study, to investigate the relevance of these proteins to cancer progression, we performed screening for functions of these proteins against growth, migration, and invasion of colon cancer cells using siRNA knock-down. Consequently, we found two proteins that were significantly suppressed viability of cancer cells. SIGMAR1 is known to participate in ER stress regulation. Treatment of colon cancer cells with a SIGMAR1 agonist mitigated tunicamycin-induced ER stress. We assumed that SIGMAR1 facilitated cancer progression via suppression of ER stress. GGT5 is an enzyme metabolizing glutathione. Knock-down of GGT5 significantly reduced growth of colon cancer cells but not normal cells. Thus, GGT5 is likely to contribute the exploitation of therapeutic methods of colorectal cancer.

Free Research Field

疾患オミクス