Development of magnetite nanoparticle with stealth and active targeting function

2016 Fiscal Year Final Research Report

• Project/Area Number: 26430154
• Research Category: Grant-in-Aid for Scientific Research (C)
• Allocation Type: Multi-year Fund
• Section: 一般
• Research Field: Tumor therapeutics
• Research Institution: Tohoku University
• Principal Investigator: Ishii Keiko 東北大学, 医学系研究科, 准教授 (00291253)
• Co-Investigator(Renkei-kenkyūsha): KAWAKAMI Kazuyoshi 東北大学, 大学院医学系研究科, 教授 (10253973) ; OSANAI Makoto 東北大学, 大学院医学系研究科, 准教授 (90286419)
• Project Period (FY): 2014-04-01 – 2017-03-31
• Keywords: ステルス化 / 酸化鉄ナノ粒子 / MRIによる診断 / 温熱療法 / ガン組織指向性 / scFv

Outline of Final Research Achievements

This study aimed to develop magnetic iron-oxide nanoparticles with novel properties for the imaging and thermal therapies of cancer in deep tissues and intractable cancer. It has been a problem that intravenously injected nanoparticles are mostly trapped by the reticuloendothelial system in liver and spleen before they reach target cancer cells. We approached this issue by coating the iron-oxide particles with RolA, a surface hydrophobin of Aspergillus oryzae. RolA-coated iron-oxide nanoparticles were less recognized by macrophages, suggesting they are endowed with stealth property. We further aimed to add an active targeting property to the iron-oxide nanoparticles and produced a single-chain fragment variable (scFv) containing variable regions of anti-HER2 antibody pertuzumab.

Free Research Field

感染免疫学