2017 Fiscal Year Final Research Report
Development of novel anti-tumor therapy target mRNA cleavage using a new method
| Project/Area Number |
26430157
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| Research Category |
Grant-in-Aid for Scientific Research (C)
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| Allocation Type | Multi-year Fund |
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| Section | 一般 |
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| Research Field |
Tumor therapeutics
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| Research Institution | Niigata University |
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Principal Investigator |
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| Co-Investigator(Kenkyū-buntansha) |
梨本 正之 新潟薬科大学, 健康・自立総合研究機構, 教授 (30228069)
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| Project Period (FY) |
2014-04-01 – 2018-03-31
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| Keywords | small guide RNA / TRUE gene silencing / 白血病 |
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| Outline of Final Research Achievements |
In order to use the TRUE gene silence method using sgRNA for treatment of hematologic malignancies, sgRNA was prepared using various tumor - associated mRNA as a target, and suppression of cell proliferation and apoptosis induction were examined. In several leukemia cell lines, the effect of sgRNA on WT1 and Bcl-2 was confirmed. It was also confirmed that this effect varied depending on the concentration of glucose in the culture medium.We could establish condition setting to predict the effect of sgRNA.
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| Free Research Field |
血液内科学
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