2017 Fiscal Year Final Research Report
Safety and persistence of genetically modified T-cells in patients with non-Hodgkin lymphoma
Project/Area Number |
26430168
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Research Category |
Grant-in-Aid for Scientific Research (C)
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Allocation Type | Multi-year Fund |
Section | 一般 |
Research Field |
Tumor therapeutics
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Research Institution | Jichi Medical University |
Principal Investigator |
OHMINE KEN 自治医科大学, 医学部, 准教授 (90316521)
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Co-Investigator(Kenkyū-buntansha) |
塚原 智典 自治医科大学, 医学部, 講師 (10362120)
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Project Period (FY) |
2014-04-01 – 2018-03-31
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Keywords | 遺伝子改変T細胞療法 / CD19抗原 / 非ホジキンリンパ腫 / 細胞疲弊化 |
Outline of Final Research Achievements |
We are conducting a clinical trial to evaluate the safety and efficacy of administering CD19 specific CAR-Ts manufactured from a patient’s autologous T-cells to combat relapsed or refractory B-cell non-Hodgkin lymphomas. We established a protocol to manufacture a sufficient number of CAR-Ts stimulated by OKT3 onto Retronectin-coated substratum. We demonstrated small scale test using patient’s blood. The patients’ T-cells showed much lower fold expansion compared to healthy donors’. The gene transfer efficiency of patient’s T-cells was equivalent to that of healthy donors. Enrollment started in December 2014. All of seven patients had successful CAR-T production, and three patients safely received an infusion. Although clinically relevant responses seen, PCR analyses failed to show the presence of CAR-Ts in one patient. There remains a need for new therapeutic modalities. These early experiences demonstrate the feasibility and significant clinical impact of CAR-T therapy.
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Free Research Field |
臨床腫瘍学
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