2016 Fiscal Year Final Research Report
Chromatin structure as a determinant of transcriptional activity
| Project/Area Number |
26430188
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| Research Category |
Grant-in-Aid for Scientific Research (C)
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| Allocation Type | Multi-year Fund |
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| Section | 一般 |
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| Research Field |
Genome biology
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| Research Institution | Fujita Health University |
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Principal Investigator |
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| Co-Investigator(Renkei-kenkyūsha) |
NIKAIDO ITOSHI 独立行政法人理化学研究所, 情報基盤センター, ユニットリーダー (00383290)
SASAGAWA YOHEI 独立行政法人理化学研究所, 情報基盤センター, 上級センター研究員 (10404344)
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| Project Period (FY) |
2014-04-01 – 2017-03-31
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| Keywords | クロマチン / ヌクレオソーム / SEVENS法 / 転写 / プロモーター / CYP19 / 次世代シーケンサー |
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| Outline of Final Research Achievements |
A controlled amount of RNA is transcribed from an individual gene. To elucidate contribution of chromatin structure to this quantitative control, the CYP19 gene, which harbors three promoters with a different activity, was chosen as a model gene. The SEVENS assay revealed that local density of nucleosomes in the promoter regions was inversely correlated to transcriptional activity of each promoter of the CYP19 gene. Such correlation was confirmed by a whole genome analysis in which DNA recovered from the SEVENS fractions was sequenced using next generation sequencing technology.
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| Free Research Field |
分子生物学
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