High-throughput sequencing method of the KIR haplotype for clinical applications

2016 Fiscal Year Final Research Report

• Project/Area Number: 26430195
• Research Category: Grant-in-Aid for Scientific Research (C)
• Allocation Type: Multi-year Fund
• Section: 一般
• Research Field: Medical genome science
• Research Institution: Kanazawa University
• Principal Investigator: Hosomichi Kazuyoshi 金沢大学, 医学系, 准教授 (50420948)
• Research Collaborator: YABE Toshio 日本赤十字社, 関東甲信越ブロック血液センター, 検査開発二係長
• Project Period (FY): 2014-04-01 – 2017-03-31
• Keywords: NGS / KIR / HLA / 造血幹細胞移植

Outline of Final Research Achievements

Killer-cell immunoglobulin-like receptors (KIRs) expressing on natural killer (NK) cells are ligands of human leukocyte antigen (HLA) class I molecules. The number of KIR genes is different among KIR haplotypes, therefore each individual has a different number of inhibitory and activating KIR genes. Here, we established high-throughput sequencing method to identify the haplotype structure of HLA and KIR genes. The method for KIR and HLA typing was based on sequence capture method with custom oligo probes and MiSeq. Sequence reads from seventeen KIR genes could be aligned. The alignment result of KIR genes has distinct depth indicating copy number variation (CNV). The single nucleotide variants were used to estimate KIR allele sequence. Combination of CNV and SNVs as KIR allele could be available to estimate KIR haplotype structure.

Free Research Field

ゲノム情報学