Identification and collection of actinomycete promoter sequences by TSS-seq

2016 Fiscal Year Final Research Report

• Project/Area Number: 26430201
• Research Category: Grant-in-Aid for Scientific Research (C)
• Allocation Type: Multi-year Fund
• Section: 一般
• Research Field: System genome science
• Research Institution: National Institute of Infectious Diseases
• Principal Investigator: Ishikawa Jun 国立感染症研究所, 真菌部, 室長 (40202957)
• Project Period (FY): 2014-04-01 – 2017-03-31
• Keywords: 転写開始点 / ゲノム工学 / 抗生物質

Outline of Final Research Achievements

Recently, discovery and development of novel antibiotics from actinomycetes is performed by genomic approaches. However there are still not enough technology for engineering antibiotic-producing actinomycetes, especially promoter for gene expression. In this work, by using NGS, we identified many transcriptional start sites (TSSs) in a Streptomyces genome and estimated putative consensus sequences of streptomyces promoter. From 18-hr culture, E. coli's Pribnow-like hexamers were found at surrounding -14 position. These findings may contribute to genomic engineering in actinomycetes,including the development of novel antibiotics.

Free Research Field

微生物ゲノム科学