Genome-wide screening of the non-flagellar genes involved in regulation of the flagellar biosynthesis and function in Escherichia coli and Salmonella

2016 Fiscal Year Final Research Report

• Project/Area Number: 26440006
• Research Category: Grant-in-Aid for Scientific Research (C)
• Allocation Type: Multi-year Fund
• Section: 一般
• Research Field: Molecular biology
• Research Institution: Okayama University
• Principal Investigator: KUTSUKAKE Kazuhiro 岡山大学, 自然科学研究科, 教授 (90143362)
• Research Collaborator: MIYAGAWA Akira 岡山大学, 大学院自然科学研究科, 大学院生
• Project Period (FY): 2014-04-01 – 2017-03-31
• Keywords: 分子遺伝学 / 遺伝子発現 / 鞭毛レギュロン / グローバル制御 / 制御ネットワーク / 鞭毛回転 / 網羅的検索

Outline of Final Research Achievements

This study was carried out to identify all the E. coli non-flagellar genes which are potentially involved in regulation of flagellar biosynthesis and function. It was found that nearly 10% of the genes have negative or positive effects on flagellar gene expression. Most of them were shown to regulate the expression of the flagellar genes via transcriptional control of the class 1 flagellar operon. Newly identified six genes were shown to encode negative regulators specific for transcription of the class 2 flagellar operons. Interestingly, some genes were found to exert negative effects on flagellar function without significant effects on flagellar gene expression.

Free Research Field

生物学