A novel regulatory mechanism for transcription of ribosomal RNA in budding yeast

2017 Fiscal Year Final Research Report

• Project/Area Number: 26440009
• Research Category: Grant-in-Aid for Scientific Research (C)
• Allocation Type: Multi-year Fund
• Section: 一般
• Research Field: Molecular biology
• Research Institution: Tokyo University of Agriculture
• Principal Investigator: Kasahara Koji 東京農業大学, 生命科学部, 准教授 (40304159)
• Project Period (FY): 2014-04-01 – 2018-03-31
• Keywords: 転写 / リボソーム / 出芽酵母 / 遺伝子発現

Outline of Final Research Achievements

Fpr1/FKBP12 make complex with rapamycin, a medically important immunosuppressive drug. This complex binds to and inhibits the kinase activity of TORC1, a key regulator of cellular responses to nutritional environment, thereby prohibiting a synthesis of ribosomal components.　However, function(s) of Fpr1 in the natural cellular-condition without the drug has been unknown. In this study, we identified fpr1 mutation that showed synthetic lethality with the deletion mutant of HMO1 gene, which is involved in a coordinated synthesis of ribosomal components. As the results of genome wide ChIP-seq analysis and the ChIP analysis using variously modified promoters, we demonstrated that Fpr1 bound to the promoter of ribosomal protein genes dependently on Rap1, a master transcriptional regulator of those genes.

Free Research Field

分子生物学