Regulation of SUMOylation dependent chromatin remoceling in heart development

2016 Fiscal Year Final Research Report

• Project/Area Number: 26440015
• Research Category: Grant-in-Aid for Scientific Research (C)
• Allocation Type: Multi-year Fund
• Section: 一般
• Research Field: Molecular biology
• Research Institution: Osaka University (2016); National Institute of Information and Communications Technology (2014-2015)
• Principal Investigator: OGAWA Hidesato 大阪大学, 生命機能研究科, 特任准教授(常勤) (20370132)
• Co-Investigator(Renkei-kenkyūsha): TAKEUCHI Jun 東京大学, 分子細胞生物学研究所, 准教授 (10451999)
• Research Collaborator: TSUCHIYA Megumi 大阪大学, 生命機能研究科, 特任助教 (00390691)
• Project Period (FY): 2014-04-01 – 2017-03-31
• Keywords: クロマチン構造変換 / 核内受容体 / 転写因子 / オートファジー

Outline of Final Research Achievements

Transcriptional coregulators contribute to several processes involving nuclear receptor transcriptional regulation. The transcriptional coregulator androgen receptor-interacting protein 4 (ARIP4) interacts with nuclear receptors and regulates their transcriptional activity. In this study, we identified p62 as a major interacting protein partner for ARIP4 in the nucleus. Nuclear magnetic resonance analysis demonstrated that ARIP4 interacts directly with the ubiquitin-associated (UBA) domain of p62. ARIP4 and ubiquitin both bind to similar amino acid residues within UBA domains; therefore, these proteins may possess a similar surface structure at their UBA-binding interfaces. We also found that p62 is required for the regulation of ARIP4 protein levels under nutrient starvation conditions. We propose that p62 is a novel binding partner for ARIP4, and that its binding regulates the cellular protein level of ARIP4 under conditions of metabolic stress.

Free Research Field

分子生物学