Analysis of molecular association and dissociation mechanism in replication restart

2016 Fiscal Year Final Research Report

• Project/Area Number: 26440031
• Research Category: Grant-in-Aid for Scientific Research (C)
• Allocation Type: Multi-year Fund
• Section: 一般
• Research Field: Structural biochemistry
• Research Institution: Kyushu University
• Principal Investigator: Abe Yoshito 九州大学, 薬学研究院, 准教授 (60315091)
• Research Collaborator: UEDA Tadashi 九州大学, 大学院薬学研究院, 教授 ; SHIROISHI Mitsunori 九州大学, 大学院薬学研究院, 助教
• Project Period (FY): 2014-04-01 – 2017-03-31
• Keywords: 複製再開始プライモソーム / タンパク質-DNA複合体 / タンパク質構造 / PriC / PriB / DnaT / DnaB

Outline of Final Research Achievements

In DNA replication restart, systematic association and dissociation of several proteins are occurred on DNA. In this application, we performed the structure and function analysis of the proteins (PriA、PriB、DnaT、PriC、DnaB ) involved in replication restart in Escherichia coli. In DnaT, we examined the oligomeric state of the N-terminal domain and the ssDNA (singles-stranded DNA) dissociation activity from PriB-ssDNA (singles-stranded DNA) complex by the linker region between the N-terminal and C-terminal domains, which is involved in the binding to PriB. Moreover, we examined the interaction between PriC and DnaB. Together with these results, we contributed elucidating the mechanism in replication restart.

Free Research Field

構造生物学、タンパク質化学