2017 Fiscal Year Final Research Report
X-ray and neutron crystallographic structure of copper amine oxidase
| Project/Area Number |
26440037
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| Research Category |
Grant-in-Aid for Scientific Research (C)
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| Allocation Type | Multi-year Fund |
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| Section | 一般 |
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| Research Field |
Structural biochemistry
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| Research Institution | Osaka Medical College |
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Principal Investigator |
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| Co-Investigator(Kenkyū-buntansha) |
林 秀行 大阪医科大学, 医学部, 教授 (00183913)
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| Project Period (FY) |
2014-04-01 – 2018-03-31
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| Keywords | 中性子結晶構造解析 / X線結晶構造解析 / トパキノン |
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| Outline of Final Research Achievements |
Copper amine oxidases (CAOs) catalyze the oxidation of various aliphatic amines to the corresponding aldehydes, ammonia and hydrogen peroxide. We focused on the structural insights into the catalytic mechanism of a CAO from Arthrobacter globiformis (AGAO) using an ultra-high resolution X-ray and neutron crystallography. We determined the structure of oxidized-form (substrate-free form) of AGAO at 1.72/1.14 angstrom by neutron/X-ray crystallography. Based on the precise three-dimensional structure information obtained, we got new knowledge on reaction mechanism accompanied by structural change and fluctuation of AGAO. Further, we also obtained the neutron and X-ray diffraction data of semiquinone intermediate. We are currently refining the structure.
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| Free Research Field |
タンパク質科学
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