2016 Fiscal Year Final Research Report
Study on the physiological significance and the molecular mechanism of Na+-pumping rhodopsin
| Project/Area Number |
26440042
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| Research Category |
Grant-in-Aid for Scientific Research (C)
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| Allocation Type | Multi-year Fund |
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| Section | 一般 |
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| Research Field |
Functional biochemistry
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| Research Institution | Hokkaido University |
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Principal Investigator |
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| Project Period (FY) |
2014-04-01 – 2017-03-31
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| Keywords | ロドプシン / ナトリウムポンプ / フォトサイクル / レチナール / 膜蛋白質 |
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| Outline of Final Research Achievements |
Microbial rhodopsins are photoactive membrane proteins containing the chromophore retinal. Upon light absorption, they undergo the cyclic photoreactions and exert respective functions such as various ion transports and the light signal transductions. Here, we performed functional analyses of the outward Na+ pumping rhodopin (NaR). The results are summarized as follows: 1) There exists a cation binding site on the NaR surface. The cation binding distorts the protein structure in the dark state, and then influences the early intermediates formed after photoexcitation. 2) Na+ uptake and release reactions were directly detected by the photo-induced changes of Na+ concentration. These reactions occurred almost simultaneously with the formation and decay of O intermediate, respectively.
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| Free Research Field |
生物物理学
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