Mechanisms of large cargoes secretion

2017 Fiscal Year Final Research Report

• Project/Area Number: 26440046
• Research Category: Grant-in-Aid for Scientific Research (C)
• Allocation Type: Multi-year Fund
• Section: 一般
• Research Field: Functional biochemistry
• Research Institution: The University of Tokyo
• Principal Investigator: SAITO KOTA 東京大学, 大学院薬学系研究科(薬学部), 助教 (60549632)
• Co-Investigator(Renkei-kenkyūsha): KATADA TOSHIAKI 武蔵野大学, 薬学部, 教授 (10088859)
• Project Period (FY): 2014-04-01 – 2018-03-31
• Keywords: 分泌 / 小胞体 / コラーゲン

Outline of Final Research Achievements

The mechanisms of collagen secretion have not been well characterized, because collagens synthesized within the ER are too large to fit into conventional COPII-coated vesicles. We previously characterized TANGO1 as a collagen cargo receptor. In this study, we have characterized that TANGO1 forms macromolecular complexes with cTAGE5 and TANGO1. In addition, we have revealed that TANGO1 acts as a scaffold in corporation with Sec16 and functions as an organizer of ER exit site. We have also identified Sec23a as a protein up-regulated during hepatic stellate cell activation. When Sec23a is depleted, activation of hepatic stellate cells is attenuated.

Free Research Field

機能生物化学