Genome-wide screening of endocytic pathway using fluorescence imaging

2017 Fiscal Year Final Research Report

• Project/Area Number: 26440067
• Research Category: Grant-in-Aid for Scientific Research (C)
• Allocation Type: Multi-year Fund
• Section: 一般
• Research Field: Functional biochemistry
• Research Institution: Tokyo University of Technology (2015-2017); Waseda University (2014)
• Principal Investigator: TOSHIMA JUNKO 東京工科大学, 医療保健学部, 教授 (00431552)
• Co-Investigator(Kenkyū-buntansha): 十島 二朗 東京理科大学, 基礎工学部生物工学科, 教授 (00333831)
• Project Period (FY): 2014-04-01 – 2018-03-31
• Keywords: エンドサイトーシス / 細胞内輸送

Outline of Final Research Achievements

Endocytosis is the process to internalize extracellular materials by clathrin coated vesicles (CCV). After internalization, CCV is transported to lysosome via early and late endosomes. However, how these processes are regulated remains unclear. In the previous work, we generated new fluorescence marker for endocytosis in budding yeast, and screened mutants that display endocytic defects. As a result, we identified many mutants in various endocytic steps. In this study, we categorized these mutants into 4 groups according to their phenotypes, and performed detailed analysis.

Free Research Field

細胞生物学