Measurement of the whole potential energy in the rotary motor F1

2016 Fiscal Year Final Research Report

• Project/Area Number: 26440082
• Research Category: Grant-in-Aid for Scientific Research (C)
• Allocation Type: Multi-year Fund
• Section: 一般
• Research Field: Biophysics
• Research Institution: Waseda University
• Principal Investigator: Adachi Kengo 早稲田大学, 理工学術院, 主任研究員(研究院准教授) (60370128)
• Project Period (FY): 2014-04-01 – 2017-03-31
• Keywords: トルク計測 / 磁気ピンセット / 回転分子モーター / ポテンシャルエネルギー / 燐酸解離

Outline of Final Research Achievements

Molecular motors in living organisms work with the hydrolysis energy of nucleotide substrates. In fact, with regard to ATP-driven molecular motors, it is thought that they move by sequentially generating force according to each chemical state such as ATP, ADP・Pi, ADP, Pi. Here, I tried to measure at single-molecule level potential energies that are viewed as the design drawing of the force generation. Specifically, using a rotary molecular motor F1-ATPase, the rotation was manipulated with magnetic tweezers while directly observing the bound nucleotides. Torque in a chemical state of one ADP binding was measured and the potential energy was obtained from the integration of torque.

Free Research Field

生物物理学