Molecular mechanism and biological function of yki mRNA foci formation in Drosophila

2016 Fiscal Year Final Research Report

• Project/Area Number: 26440097
• Research Category: Grant-in-Aid for Scientific Research (C)
• Allocation Type: Multi-year Fund
• Section: 一般
• Research Field: Cell biology
• Research Institution: Kyoto Institute of Technology
• Principal Investigator: Yoshida Hideki 京都工芸繊維大学, 応用生物学系, 助教 (30570600)
• Research Collaborator: UMEGAWACHI Takanari
• Project Period (FY): 2014-04-01 – 2017-03-31
• Keywords: yorkie / mRNAの小胞体標的化 / ショウジョウバエ / SRP非依存的 / 翻訳抑制 / ステムループ

Outline of Final Research Achievements

We revealed that yorkie (yki) mRNA is localized to the P-bodies, which is ribonucleoprotein complexes for translational repression and exist adjacent to the ER, depending on a secondary RNA structure in its own 3’ untranslated region (UTR). Furthermore, we showed that the transgenic flies expressing the yorkie mRNA deleted the secondary RNA structure exhibited the enlarged compound eye phenotype. Further analyses using this fly line indicated that the secondary RNA structure in yki 3’UTR has an important role for translational repression in vivo. For the regulation of yki functional expression, importance of post-translational regulation of Yorkie by phosphorylation has been well established. However, in this study, we suggested another novel regulation which has similarly strong impact on yki gene expression.

Free Research Field

mRNAの細胞内局在