Post-translational regulation of Hes1 in nerual stem cells

2016 Fiscal Year Final Research Report

• Project/Area Number: 26440122
• Research Category: Grant-in-Aid for Scientific Research (C)
• Allocation Type: Multi-year Fund
• Section: 一般
• Research Field: Developmental biology
• Research Institution: Kyoto University
• Principal Investigator: Kobayashi Taeko 京都大学, ウイルス・再生医科学研究所, 助教 (40402804)
• Project Period (FY): 2014-04-01 – 2017-03-31
• Keywords: Hes1 / オシレーション / 翻訳後修飾 / 幹細胞分化

Outline of Final Research Achievements

Hes1 regulates the maintenance and the differentiation of stem cells by its　dynamic expression. Previous studies demonstrated that Hes1 expression　oscillates in stem cells, and that the oscillation contributes to their potency　and differentiation fates. However, the molecular mechanisms regulating the　oscillatory expression of Hes1 protein have not been fully identified.
We have focused on the post-translational regulation of Hes1 protein and tried　to identify regulatory factors from the binding partners of Hes1 protein. We　identified Usp27x and its homologs as new deubiquitinases (DUBs) of Hes1. We　reported that these DUBs stabilize and modulate Hes1 protein dynamics by　removing ubiquitin molecules, and thereby regulate neuronal differentiation of　stem cells (Kobayashi et al, 2015). Moreover, we identified some amino acid　residues crucial for Hes1 functions by post-translational regulation. We also　identified new factors bound to recombinant Hes1.

Free Research Field

発生生物学