Comprehensive analysis of Arabidopsis myosin XIs

2016 Fiscal Year Final Research Report

• Project/Area Number: 26440131
• Research Category: Grant-in-Aid for Scientific Research (C)
• Allocation Type: Multi-year Fund
• Section: 一般
• Research Field: Plant molecular biology/Plant physiology
• Research Institution: Chiba University
• Principal Investigator: Kohji Ito 千葉大学, 大学院融合科学研究科, 教授 (50302526)
• Project Period (FY): 2014-04-01 – 2017-03-31
• Keywords: ミオシン / アクチン / 原形質流動 / 分子モーター / シロイヌナズナ

Outline of Final Research Achievements

Arabidopsis possesses 13 genes coding myosin XIs, which operate in the intracellular transport systems, such as cytoplasmic streaming, vesicular trafficking, organelles trafficking, and tension maintenance. Because fundamental data for molecular properties are lacking, physiological functions of each myosin XIs are poorly understood. Therefore we have taken a comprehensive analysis of molecular properties of 13 Arabidopsis myosin XIs. Pollen-specific myosin XIs showed high actin-activated ATPase and high velocities. Ubiquitously expressed myosin XIs showed similar velocity as that of cytoplasmic streaming. Nuclear membrane specific myosin XI (myosin XI-I) showed very low actin-activated ATPase and low velocity. These results demonstrated that angiosperm increased numbers of myosin XIs and gave specific functions on myosin XIs during the evolution to sustain highly complicated biological system.

Free Research Field

植物生理学