2016 Fiscal Year Final Research Report
Regulation of the amount of translation products in chloroplasts
| Project/Area Number |
26440136
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| Research Category |
Grant-in-Aid for Scientific Research (C)
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| Allocation Type | Multi-year Fund |
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| Section | 一般 |
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| Research Field |
Plant molecular biology/Plant physiology
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| Research Institution | Nagoya University |
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Principal Investigator |
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| Co-Investigator(Renkei-kenkyūsha) |
NAKAMURA Masayuki 名古屋大学, 遺伝子実験施設, 研究員 (60322145)
YUKAWA Maki 名古屋市立大学, システム自然科学研究科, 研究員 (00448705)
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| Project Period (FY) |
2014-04-01 – 2017-03-31
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| Keywords | 葉緑体 / 翻訳 / mRNA / リボソーム / in vitro系 / 翻訳抑制 / 上流ORF |
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| Outline of Final Research Achievements |
Chloroplast ribosomes include 59 proteins in equimolar ratios. Though their sizes vary significantly, these proteins should be synthesized as equal moles. The translation elongation rates of a large S2 mRNA and a small S16 mRNA coding regions are found to be similar. However, the translation initiation rate of S16 mRNA 5'-UTR was very low. This is due to the presence of an S16-specific translation repressor in a chloroplast soluble fraction. We propose a model that translation of ribosome protein synthesis is regulated by mRNA-specific factors.
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| Free Research Field |
生物学
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