2018 Fiscal Year Final Research Report
Dissection of mechanisms regulating the outermost-cell specific gene expression
Project/Area Number |
26440142
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Research Category |
Grant-in-Aid for Scientific Research (C)
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Allocation Type | Multi-year Fund |
Section | 一般 |
Research Field |
Plant molecular biology/Plant physiology
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Research Institution | Osaka University |
Principal Investigator |
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Research Collaborator |
IIDA Hiroyuki
YOSHIDA Ayaka
TAKADA Nozomi
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Project Period (FY) |
2014-04-01 – 2019-03-31
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Keywords | 表皮分化 / マスター転写因子 / シロイヌナズナ / 転写後調節 |
Outline of Final Research Achievements |
The leaves and stems of land plants are covered with a single layer of epidermis. The epidermis plays an important role in protecting plants from drought and pathogen infection. However, molecular mechanisms promoting epidermal cell differentiation only in the outermost cells is not well understood. We found that the ATML1 protein, a master regulator of epidermal cell fate, accumulated only in the outermost cells. In addition, nuclear accumulation of ATML1 was reduced in the inner cells compared with outermost cells in transgenic plants constitutively expressing ATML1, suggesting that post-transcriptional repressions are required for the outermost-cell specific activity of ATML1. Furthermore, we have identified several amino acid sequences that are required to reduce nuclear accumulation of ATML1 in the inner cells. We have also found candidate genes that promote or suppress the activity of ATML1.
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Free Research Field |
植物発生生物学
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Academic Significance and Societal Importance of the Research Achievements |
発生中の植物細胞はフレキシブルに細胞運命を変化させる。それぞれの細胞がその最終的な位置に応じてどの細胞タイプへと分化するかを決めるのが、植物の発生の特徴である。しかし、植物細胞がどうやって自分の位置を認識しているのかはあまり理解されていない。本研究は、細胞の置かれた位置に応じてATML1タンパク質の核への蓄積が変化することを示したものであり、植物細胞が位置を認識して分化するメカニズムの一端が解明できた。また、ATML1の活性を調節する原理が明らかになったことから、乾燥に強い多層表皮を持つ植物の作出も可能かもしれない。さらに、陸上植物が表皮を獲得した進化の過程の理解につながることも期待できる。
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