Stomatal response in crassulacean acid metabolism plants

2016 Fiscal Year Final Research Report

• Project/Area Number: 26440150
• Research Category: Grant-in-Aid for Scientific Research (C)
• Allocation Type: Multi-year Fund
• Section: 一般
• Research Field: Plant molecular biology/Plant physiology
• Research Institution: Kyushu University
• Principal Investigator: Doi Michio 九州大学, 基幹教育院, 助教 (00167537)
• Project Period (FY): 2014-04-01 – 2017-03-31
• Keywords: CAM植物 / 気孔 / 青色光応答 / Ｃ3植物 / 光合成

Outline of Final Research Achievements

We investigated stomatal blue light (BL) response in obligate crassulacean acid metabolism (CAM) plants, Kalanchoe pinnata and Kalanchoe daigremontiana. The stomatal responses to BL were determined in both intact leaves and detached epidermis using dual-beam protocol to distinguish the BL-dependent stomatal opening from the photosynthesis-dependent stomatal opening. The stomata open in response to BL superimposed on the background red light (RL). The BL-dependent stomatal opening was completely inhibited by both an inhibitor of type 1 protein phosphatase and an inhibitor of the plasma membrane H+-ATPase. A fungous toxin fusicoccin (FC), an activator of the plasma membrane H+-ATPase induced stomatal opening in the dark. BL and FC caused both activation of the plasma membrane H+-ATPase in guard cells, and stomatal opening.
These results indicate that BL-dependent stomatal opening operates in obligate CAM plants, regardless of the difference in the modes of photosynthesis.

Free Research Field

植物生理学