Mechanism of enhanced salinity tolerance by an endogenouse peptide elicitor in Arabidopsis

2016 Fiscal Year Final Research Report

• Project/Area Number: 26440153
• Research Category: Grant-in-Aid for Scientific Research (C)
• Allocation Type: Multi-year Fund
• Section: 一般
• Research Field: Plant molecular biology/Plant physiology
• Research Institution: Osaka Prefecture University
• Principal Investigator: Yamaguchi Yube 大阪府立大学, 生命環境科学研究科, 准教授 (60335487)
• Research Collaborator: DOMAN Kohei 北海道大学, 大学院・農学院, 学生 ; FUJINO Kaien 北海道大学, 大学院・農学研究院, 准教授 (80229020) ; MURAKAMI Yuhei 大阪府立大学, 生命環境科学研究科, 学生 ; FUJII Kenta 大阪府立大学, 生命環境科学域, 学生
• Project Period (FY): 2014-04-01 – 2017-03-31
• Keywords: plant peptide elicitor / DAMP / 耐塩性 / シロイヌナズナ

Outline of Final Research Achievements

We investigated the mechanism of enhanced salinity tolerance in Arabidopsis thaliana overexpressing AtproPep1, a precursor protein of 23-amino acid peptide AtPep1. Amount of AtproPep1 protein in overexpression lines was increased in response to NaCl treatment. Expression of 2 matacaspase genes and SOS genes, which were predicted to process AtproPep1 and important for Na+ exclusion from the cells,respectively, were higher in the overexpression lines than in WT. Since Na+ content was kept lower in the overexpression lines under NaCl stress, we are speculating that promotion of AtPep1 generation by NaCl treatment in the AtproPep1 overexpression lines led to activation of Na+ exclusion from cells, which are directly related to enhanced salt tolerance.

Free Research Field

植物分子生物学