Analysis on molecular mechanism of protein transport to peroxisomes

2017 Fiscal Year Final Research Report

• Project/Area Number: 26440157
• Research Category: Grant-in-Aid for Scientific Research (C)
• Allocation Type: Multi-year Fund
• Section: 一般
• Research Field: Plant molecular biology/Plant physiology
• Research Institution: National Institute for Basic Biology
• Principal Investigator: Mano Shoji 基礎生物学研究所, 多様性生物学研究室, 准教授 (20321606)
• Co-Investigator(Renkei-kenkyūsha): NISHIMURA MIKIO 基礎生物学研究所, 名誉教授 (80093061) ; KANAI MASATAKE 基礎生物学研究所, 多様性生物学研究室, NIBBリサーチフェロー (30611488) ; OIKAWA KAZUSATO 理化学研究所, 環境資源科学研究センター, 研究員 (70508457) ; KAMIGAKI AKANE 基礎生物学研究所, 多様性生物学研究室, 特別協力研究員 (30399315)
• Research Collaborator: KONDO MAKI ; GOTO SHINO ; HIKINO KAZUMI ; NAGATA KYOKO ; YAMAGUCHI CHINAMI ; NAKAGAWA TSUYOSHI ; KOUCHI TAKAYUKI ; NISHIHAMA RYUICHI ; YAMATO KATSUYUKI
• Project Period (FY): 2014-04-01 – 2018-03-31
• Keywords: ペルオキシソーム / シロイヌナズナ / apem変異体 / タンパク質輸送 / Peroxin (PEX) / GFP / オルガネラ / ゼニゴケ

Outline of Final Research Achievements

From imaging analysis using transgenic Marchantia polymorpha expressing PTS1 (Peroxisome targeting signal 1)- or PTS2-Citrine and immunoelectron microscopic analysis revealed the presence of PTS1- and PTS2-dependent protein transport in M. polymorpha. Bioinformatics analysis identified the orthologous genes in M. polymporpha genome. Using this information, we succeeded in generation of some mutants whose peroxisomal genes were disrupted by the CRISPR/Cas9 method. In addition, we generated the Gateway technology-compatible binary vectors, and these vectors functioned in M. polymorpha cells.
From the analysis of one of Arabidopsis apem (aberrant peroxisome morphology), the ubiquitin signaling pathway is involved in protein transport to peroxisomes, and that the defective of ubiquitin-dependent protein transport causes normal peroxisome functions, such as lipid metabolism and photorespiration.

Free Research Field

植物分子生物学