2016 Fiscal Year Final Research Report
Analysis of interaction emergence and disappearance mechanism by experimantal evolution
Project/Area Number |
26440200
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Research Category |
Grant-in-Aid for Scientific Research (C)
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Allocation Type | Multi-year Fund |
Section | 一般 |
Research Field |
Evolutionary biology
|
Research Institution | Toho University |
Principal Investigator |
|
Project Period (FY) |
2014-04-01 – 2017-03-31
|
Keywords | 実験室進化 / 高温適応進化 / 相互作用 / 大腸菌 |
Outline of Final Research Achievements |
On the early process of E. coli 45℃ adaptive evolution, we have observed emergence and disappearance of interaction dependent growth. In this study, we first isolated E. coli clones with mutations correspond to thermo-adaptive evolution, and analyzed metabolic features (interaction dependence of growth, oxygen consumption, glucose consumption, and lactate production) of every mutant at 45℃. The results indicated adaptation pathway during 45℃ as following; 1st: alteration of mode of respiration, 2nd: accumulation of metabolites in growth media, 3rd: emergence of mutant with ability of utilization of metabolites as interactor, 4th: stabilization of intracellular environment with chaperonin mutation, 5th: recovery of mode of aspiration, and finally disappearance of interaction.
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Free Research Field |
分子細胞進化学
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